A few years later a much more sophisticated and improved version of this proposal was performed by a research group at the University of Milan, utilizing neurons derived from the same human neural stem cells, cultured on microelectrode arrays (MEAs) contained in 2 cm dia basins inside Faraday cages, utilizing low energy lasers to stimulate the neurons (Pizzi et al, 2004a; 2004b; 2005). For the purposes of their experiments, they usually have 2 of these neuronal basins separated by 20 cm or more. The voltages in these neuronal basins prior to laser stimulation are 5 mV peak to peak. Laser stimulation of just one of the basins at 670 nm, naturally results in an electrical signal being generated by this basin, of a frequency directly related to the frequency of the laser input, which can vary from 0-2,000 Hz and, with a peak to peak amplitude of 20 mV.
There is a normal delay between the time of activation of the laser, its impingement upon the neurons and the resulting electrical signal, of some 300 ms. What they have found most interesting over a period of several years and thousands of laser pulses, is that the separated non-stimulated basin displays simultaneous or correlated electrical signals, although of a reduced amplitude in mV, and that there is a simultaneity or correlation in
the 2 basins frequencies, between 500-2,000 Hz, with a sharp common peak around 900 Hz, implying biological entanglement and nonlocality. Periodograms of the two signals are also about coincident.
They have resorted to every possible technique (as detailed in their papers) to rule out the possibility that this effect might be due to some error in their experimental protocol, in equipment malfunction or some type of human intervention, since this implies nonlocality, even though the 2 neuronal basins are separated by only a few cm. To show you the extent they have gone to, to rule out any possible local or classical explanation, they first start out with just one neuronal basin and 2 control basins, without any neurons in them but, containing either their culture liquid or matrigel. Both basins are inside Faraday cages. They stimulate the main basin containing the human neurons on the MEAs with the laser, and get the usual electrical signal response from it but, there is never any electrical signals or response from either of the two control basins.
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